Recent advances in thiol and sulfide reactive probes

J Cell Biochem. 2014 Jun;115(6):1007-22. doi: 10.1002/jcb.24762.

Abstract

Because of the biological relevance of thiols and sulfides such as cysteine, homocysteine, glutathione and hydrogen sulfide, their detection has attracted a great deal of research interest. Fluorescent probes are emerging as a new strategy for thiol and hydrogen sulfide analysis due to their high sensitivity, low cost, and ability to detect and image thiols in biological samples. In this short review, we have summarized recent advances in the development of thiol and hydrogen sulfide reactive fluorescent probes. These probes are compared and contrasted with regard to their designing strategies, mechanisms, photophysical properties, and/or reaction kinetics. Biological applications of these probes are also discussed.

Keywords: CYSTEINE; FLUORESCENT PROBES; GLUTATHIONE; HOMOCYSTEINE; HYDROGEN SULFIDE; THIOL DETECTION.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cysteine / analysis
  • Cysteine / chemistry
  • Fluorescent Dyes / chemistry*
  • Glutathione / analysis
  • Glutathione / chemistry
  • Homocysteine / analysis
  • Homocysteine / chemistry
  • Hydrogen Sulfide / analysis
  • Hydrogen Sulfide / chemistry
  • Models, Chemical
  • Molecular Structure
  • Sulfhydryl Compounds / analysis*
  • Sulfhydryl Compounds / chemistry
  • Sulfides / analysis*
  • Sulfides / chemistry

Substances

  • Fluorescent Dyes
  • Sulfhydryl Compounds
  • Sulfides
  • Homocysteine
  • Glutathione
  • Cysteine
  • Hydrogen Sulfide