Pseudobagrus trilineatus was listed as a vulnerable species in the China Species Red List. In the present paper, we reported the complete mitochondrial genome of P. trilineatus. The genome was 16,535 bp in length, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region, a gene organization similar to that of typical vertebrate mitochondrial genome. The overall nucleotide base composition of P. trilineatus mitogenome was A (31.56%), T (26.48%), C (27.13%) and G (14.83%), with an AT content of 58.04%. This study contributes to providing new molecular data for the conservation of this endangered species.
Keywords: Mitochondrial genome; Pseudobagrus trilineatus; sequencing.