The complete mitochondrial DNA sequence of Thymallus grubii (Salmonidae: Thymallinae) was determined in this study. The complete mtDNA sequence of T. grubii was 16,657 bp in length, consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. Intergenic spacer and overlapping sequences were found in mtDNA of T. grubii. The base composition of the whole mtDNA was 27.9% A, 27.2% T, 27.5% C and 17.4% G, with a slightly A+T bias of 55.1%. Seven protein-coding genes had an incomplete stop codon in T. grubii.
Keywords: Amur grayling; Thymallus grubii; fish; mitochondrial genome.