Abstract
Modulation of the interaction of regulatory 14-3-3 proteins to their physiological partners through small cell-permeant molecules is a promising strategy to control cellular processes where 14-3-3s are engaged. Here, we show that the fungal phytotoxin fusicoccin (FC), known to stabilize 14-3-3 association to the plant plasma membrane H(+) -ATPase, is able to stabilize 14-3-3 interaction to several client proteins with a mode III binding motif. Isothermal titration calorimetry analysis of the interaction between 14-3-3s and different peptides reproducing a mode III binding site demonstrated the FC ability to stimulate 14-3-3 the association. Moreover, molecular docking studies provided the structural rationale for the differential FC effect, which exclusively depends on the biochemical properties of the residue in peptide C-terminal position. Our study proposes FC as a promising tool to control cellular processes regulated by 14-3-3 proteins, opening new perspectives on its potential pharmacological applications.
Keywords:
14-3-3 proteins; drug discovery; fusicoccin; molecular docking; protein-protein interaction.
© 2014 International Union of Biochemistry and Molecular Biology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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14-3-3 Proteins / chemistry
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14-3-3 Proteins / metabolism*
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Binding Sites
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Calorimetry
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Cell Membrane / metabolism
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Cyclin-Dependent Kinase Inhibitor p27 / metabolism
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Gene Expression Regulation / drug effects*
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Glycosides / pharmacology*
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Humans
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Models, Molecular
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Mycotoxins / pharmacology*
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Nerve Tissue Proteins / metabolism
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Phospholipase D / metabolism
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Phosphopeptides / chemistry
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Phosphopeptides / metabolism*
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Potassium Channels, Tandem Pore Domain / metabolism
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Protein Binding
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Protein Conformation
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Protein Interaction Domains and Motifs / drug effects*
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Proton-Translocating ATPases / metabolism
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Receptors, G-Protein-Coupled / metabolism
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Receptors, Interleukin-9 / metabolism
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Receptors, Peptide / metabolism
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Thermodynamics
Substances
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14-3-3 Proteins
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CDKN1B protein, human
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GPR15 protein, human
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Glycosides
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HAP1 protein, human
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Mycotoxins
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Nerve Tissue Proteins
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Phosphopeptides
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Potassium Channels, Tandem Pore Domain
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Receptors, G-Protein-Coupled
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Receptors, Interleukin-9
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Receptors, Peptide
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Cyclin-Dependent Kinase Inhibitor p27
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potassium channel subfamily K member 3
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fusicoccin
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phospholipase D delta
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Phospholipase D
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Proton-Translocating ATPases