Structural basis for regulation of ribosomal RNA 2'-o-methylation

Abstract

Methods that interrogate different, yet overlapping structural-size ranges were integrated to gain high-resolution information on the Pyrococcus furiosus box C/D ribonucleoprotein complex. The new structure shows how 2'-O-methylation of RNA substrates through four active methyltransferases occurs in a strictly sequential manner.

Keywords: 2′-O-methylation; NMR spectroscopy; RNA modification; ribosome biogenesis; small-angle neutron scattering.