Abstract
Serum-free medium conditioned by exposure to rabbit costal chondrocytes in culture inhibited the proliferation and DNA synthesis of bovine pulmonary endothelial cells in culture. The factor in conditioned medium did not inhibit DNA synthesis in B16 melanoma cells, L1210 cells or 3T3 fibroblasts in culture, but it inhibited angiogenesis in the chorioallantoic membrane of chick embryos induced by B16 melanoma and growth of the tumor transplanted onto the membrane. These findings strongly suggest that rabbit costal chondrocytes produce an anti-angiogenesis factor that is similar to cartilage-derived anti-tumor factor (CATF).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiogenesis Inhibitors
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Animals
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Cartilage / cytology
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Cartilage / metabolism*
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Cattle
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Cell Division / drug effects*
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Cells, Cultured
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Chick Embryo
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Culture Media / analysis
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DNA Replication / drug effects*
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Depression, Chemical
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Endothelium / drug effects
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Fibroblasts / drug effects
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Leukemia L1210 / pathology
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Lung
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Melanoma, Experimental / pathology
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Mice
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Neoplasm Transplantation
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Neovascularization, Pathologic*
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Proteins / isolation & purification
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Proteins / metabolism
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Proteins / pharmacology*
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Rabbits
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Ribs
Substances
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Angiogenesis Inhibitors
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Culture Media
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Proteins