Highly flexible UV-vis radiation sources and novel detection schemes for spectrophotometric HPLC detection

Anal Chem. 2014 Jan 21;86(2):1146-52. doi: 10.1021/ac403146y. Epub 2014 Jan 7.

Abstract

The concept and performance of the first multiwavelength deep UV light-emitting-diode-based high-performance liquid chromatography (HPLC) absorbance detector are presented. In single-wavelength mode and with optical reference, the limit of detection (LOD) is comparable to conventional state-of-the-art HPLC absorbance detectors. In multiwavelength mode--at present up to eight wavelengths without optical reference--the LOD is about 10 times higher than in single-wavelength mode. Multiplexing and demultiplexing methods are used to separate chromatographic signals in multiwavelength mode and keeps the detector configuration simple and yet flexible. Depending on the operation mode, stray light is either totally negligible or controlled electronically and digitally.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthracenes / analysis*
  • Caffeine / analysis*
  • Chromatography, High Pressure Liquid / instrumentation*
  • Chromatography, High Pressure Liquid / methods
  • Limit of Detection
  • Toluene / analysis*
  • Ultraviolet Rays

Substances

  • Anthracenes
  • Toluene
  • Caffeine
  • anthracene