Upregulation of microRNA-107 induces proliferation in human gastric cancer cells by targeting the transcription factor FOXO1

FEBS Lett. 2014 Feb 14;588(4):538-44. doi: 10.1016/j.febslet.2013.12.009. Epub 2013 Dec 25.

Abstract

MicroRNA-107 (miR-107) has been demonstrated to regulate proliferation and apoptosis in many types of cancers. Nevertheless, its biological function in gastric cancer remains largely unexplored. Here, we found that the expression level of miR-107 was increased in gastric cancer in comparison with the adjacent normal tissues. The enforced expression of miR-107 was able to promote cell proliferation in NCI-N87 and AGS cells, while miR-107 antisense oligonucleotides (antisense miR-107) blocked cell proliferation. At the molecular level, our results further revealed that expression of FOXO1 was negatively regulated by miR-107. Therefore, the data reported here demonstrate that miR-107 is an important regulator in gastric cancer, which will contribute to a better understanding of the important mis-regulated miRNAs in gastric cancer.

Keywords: Cell proliferation; FOXO1; Gastric cancer; MicroRNA-107.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Cycle / genetics
  • Cell Line, Tumor
  • Cell Proliferation
  • Cyclin D1 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Cyclin-Dependent Kinase Inhibitor p27 / metabolism
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / genetics*
  • NF-kappa B / metabolism
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology*
  • Up-Regulation*

Substances

  • Cyclin-Dependent Kinase Inhibitor p21
  • FOXO1 protein, human
  • Forkhead Box Protein O1
  • Forkhead Transcription Factors
  • MIRN107 microRNA, human
  • MicroRNAs
  • NF-kappa B
  • Cyclin D1
  • Cyclin-Dependent Kinase Inhibitor p27