We report a case of gallbladder stone receiving three-dimensional (3D) laparoscopic cholecystectomy, which allowed 3D visualization of the laparoscopic operative field and faithfully displayed the 3D anatomic structures of the abdominal organs and the gallbladder triangle. The operation was successfully completed in 32 min without intraoperative complications. 3D laparoscopic surgery allows more precise operation with reduced complications and helps to shorten the operative time, and is suitable for more complex laparoscopic surgery.