Selection of oleaginous yeasts with high lipid productivity for practical biodiesel production

Bioresour Technol. 2014 Feb:153:230-5. doi: 10.1016/j.biortech.2013.11.086. Epub 2013 Dec 8.

Abstract

The lipid-accumulating ability of 500 yeast strains isolated in Japan was evaluated. Primary screening revealed that 31 strains were identified as potential lipid producers, from which 12 strains were cultivated in a medium containing 3% glucose. It was found that JCM 24511 accumulated the highest lipid content, up to 61.53%, while JCM 24512 grew the fastest. They were tentatively identified as Cryptococcus sp. and Cryptococcus musci, respectively. The maximum lipid concentration of 1.49g/L was achieved by JCM 24512. Similarly, JCM 24511 also achieved a high lipid production of 1.37g/L. High lipid productivity is the most important characteristic of oleaginous yeasts from the viewpoint of practical production. Among the strains tested here, JCM 24512 had the best lipid productivity, 0.37g/L/day. The results show that the isolated yeasts could be promising candidates for biodiesel production.

Keywords: Cryptococcus musci; Cryptococcus podzolicus; Fatty acids; Lipid productivity; Oleaginous yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biofuels / microbiology*
  • Biomass
  • Biotechnology / methods*
  • Cryptococcus / metabolism*
  • European Union
  • Fatty Acids / metabolism
  • Fatty Acids, Monounsaturated
  • Jatropha / chemistry
  • Kinetics
  • Lipids / biosynthesis*
  • Plant Oils / metabolism
  • Rapeseed Oil
  • Time Factors
  • United States

Substances

  • Biofuels
  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • Lipids
  • Plant Oils
  • Rapeseed Oil