Virus-induced signaling influences endosome trafficking, virus entry, and replication

Methods Enzymol. 2014:534:65-76. doi: 10.1016/B978-0-12-397926-1.00004-4.

Abstract

Viruses are obligate intracellular pathogens that interact with host cell machinery for enabling entry, replication, and spread. This chapter describes methods for studying the interaction of viruses with host cell signaling pathways and surface receptors during cellular infection, with an emphasis on protein kinases. We also describe how use of immunofluorescence confocal microscopy for imaging virus-host interactions provides a powerful approach for obtaining structural correlations that extend results of immunological and biochemical assays.

Keywords: Cell Signalling; Endocytosis; Virus Entry; Virus Traffickin.

MeSH terms

  • Animals
  • Blotting, Western
  • Endosomes / metabolism*
  • Endosomes / virology
  • Enzyme-Linked Immunosorbent Assay
  • Extracellular Signal-Regulated MAP Kinases / genetics
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Fibroblasts / metabolism*
  • Fibroblasts / virology
  • Gene Expression Regulation
  • Genes, Reporter
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Host-Pathogen Interactions
  • Influenza A Virus, H1N1 Subtype / physiology*
  • Mice
  • Mice, Knockout
  • Microscopy, Fluorescence
  • Myeloid Differentiation Factor 88 / deficiency
  • Myeloid Differentiation Factor 88 / genetics
  • Protein Transport
  • Respiratory Syncytial Viruses / physiology*
  • Signal Transduction / genetics*
  • Virus Internalization
  • Virus Replication
  • p38 Mitogen-Activated Protein Kinases / genetics
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Myd88 protein, mouse
  • Myeloid Differentiation Factor 88
  • Green Fluorescent Proteins
  • Extracellular Signal-Regulated MAP Kinases
  • p38 Mitogen-Activated Protein Kinases