High yield production of sugars from deproteinated palm kernel cake under microwave irradiation via dilute sulfuric acid hydrolysis

Bioresour Technol. 2014 Feb:153:69-78. doi: 10.1016/j.biortech.2013.11.055. Epub 2013 Nov 27.

Abstract

Recent years, great interest has been devoted to the conversion of biomass-derived carbohydrate into sugars, such as glucose, mannose and fructose. These are important versatile intermediate products that are easily processed into high value-added biofuels. In this work, microwave-assisted dilute sulfuric acid hydrolysis of deproteinated palm kernel cake (DPKC) was systematically studied using Response Surface Methodology. The highest mannose yield (92.11%) was achieved at the optimized condition of 148°C, 0.75N H2SO4, 10min 31s and substrate to solvent (SS) ratio (w/v) of 1:49.69. Besides that, total fermentable sugars yield (77.11%), was obtained at 170°C, 0.181N H2SO4, 6min 6s and SS ratio (w/v) of 1:40. Ridge analysis was employed to further verify the optimum conditions. Thus, this work provides fundamental data of the practical use of DPKC as low cost, high yield and environmental-friendly material for the production of mannose and other sugars.

Keywords: Mannose; Microwave-assisted hydrolysis; Palm kernel cake; RSM optimization; Ridge analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Araceae / chemistry*
  • Araceae / drug effects
  • Bioreactors
  • Biotechnology / methods*
  • Carbohydrates / biosynthesis*
  • Hydrolysis / drug effects
  • Microwaves*
  • Monosaccharides / analysis
  • Plant Proteins / isolation & purification*
  • Regression Analysis
  • Solvents
  • Sulfuric Acids / pharmacology*
  • Temperature
  • Time Factors

Substances

  • Carbohydrates
  • Monosaccharides
  • Plant Proteins
  • Solvents
  • Sulfuric Acids
  • sulfuric acid