Quantitative phase imaging via Fourier ptychographic microscopy

Opt Lett. 2013 Nov 15;38(22):4845-8. doi: 10.1364/OL.38.004845.

Abstract

Fourier ptychographic microscopy (FPM) is a recently developed imaging modality that uses angularly varying illumination to extend a system's performance beyond the limit defined by its optical components. The FPM technique applies a novel phase-retrieval procedure to achieve resolution enhancement and complex image recovery. In this Letter, we compare FPM data to theoretical prediction and phase-shifting digital holography measurement to show that its acquired phase maps are quantitative and artifact-free. We additionally explore the relationship between the achievable spatial and optical thickness resolution offered by a reconstructed FPM phase image. We conclude by demonstrating enhanced visualization and the collection of otherwise unobservable sample information using FPM's quantitative phase.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Fourier Analysis
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods*
  • Microscopy, Phase-Contrast / instrumentation*
  • Microscopy, Phase-Contrast / methods*
  • Signal Processing, Computer-Assisted / instrumentation*