Genetic association between NOD2 polymorphism and infection status by Mycobacterium avium ssp. paratuberculosis in German Holstein cattle

Anim Genet. 2014 Feb;45(1):114-6. doi: 10.1111/age.12097. Epub 2013 Dec 10.

Abstract

The aim of the study was the analysis of the nucleotide-binding oligomerization domain containing 2 (NOD2, formerly CARD15) as a candidate gene for Mycobacterium avium ssp. paratuberculosis infection in cattle. Eleven SNPs in the NOD2 gene were identified, and finally, four SNPs were included in a case-control study using 324 German Holstein cows tested for paratuberculosis using fecal culture and ELISA. The SNP (GenBank) AY518738S04:g.521G>A in exon 4 showed a significant association between the fecal culture status of the animals and NOD2 allele variants. The other three SNPs showed no associations in German Holstein cows.

Keywords: CARD15; bovine; candidate gene; fecal culture; nucleotide-binding oligomerization domain containing 2; paratuberculosis.

MeSH terms

  • Animals
  • Case-Control Studies
  • Cattle / genetics*
  • Cattle Diseases / genetics*
  • Cattle Diseases / microbiology
  • Female
  • Gene Frequency
  • Haplotypes
  • Linkage Disequilibrium
  • Molecular Sequence Data
  • Mycobacterium avium subsp. paratuberculosis
  • Nod2 Signaling Adaptor Protein / genetics*
  • Paratuberculosis / genetics*
  • Polymorphism, Single Nucleotide*

Substances

  • Nod2 Signaling Adaptor Protein

Associated data

  • GENBANK/AH013658
  • GENBANK/AY518737
  • GENBANK/JX448702