MurA as a primary target of tulipalin B and 6-tuliposide B

Biosci Biotechnol Biochem. 2013;77(12):2517-9. doi: 10.1271/bbb.130663. Epub 2013 Dec 7.

Abstract

(-)-Tulipalin B and (+)-6-tuliposide B were confirmed to inhibit MurA in vitro. However, contrary to fosfomycin, these compounds showed potent inhibitory activities against MurA overexpressing Escherichia coli, especially in the presence of UDP-GlcNAc. These observations suggest that these compounds induced bacterial cell death not through a MurA malfunction, but in such a MurA-mediated indirect manner as the inhibition of other Mur enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / pharmacology
  • Alkyl and Aryl Transferases / antagonists & inhibitors*
  • Alkyl and Aryl Transferases / genetics
  • Enzyme Inhibitors / pharmacology*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Glucosides / pharmacology*
  • Hydroxybutyrates / pharmacology*

Substances

  • 6-tuliposide B
  • Enzyme Inhibitors
  • Glucosides
  • Hydroxybutyrates
  • tulipalin B
  • Alkyl and Aryl Transferases
  • UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  • 4-Butyrolactone