Implementing homo- and heterodecoupling in region-selective HSQMBC experiments

Laura Castañar; Josep Saurí; Pau Nolis; Albert Virgili; Teodor Parella

doi:10.1016/j.jmr.2013.10.022

Implementing homo- and heterodecoupling in region-selective HSQMBC experiments

J Magn Reson. 2014 Jan:238:63-9. doi: 10.1016/j.jmr.2013.10.022. Epub 2013 Nov 15.

Authors

Laura Castañar¹, Josep Saurí¹, Pau Nolis¹, Albert Virgili¹, Teodor Parella²

Affiliations

¹ Servei de Ressonància Magnètica Nuclear and Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Catalonia), Spain.
² Servei de Ressonància Magnètica Nuclear and Departament de Química, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Catalonia), Spain. Electronic address: teodor.parella@uab.cat.

PMID: 24316187
DOI: 10.1016/j.jmr.2013.10.022

Abstract

An NMR method to enhance the sensitivity and resolution in band-selective long-range heteronuclear correlation spectra is proposed. The excellent in-phase nature of the selHSQMBC experiment allows that homonuclear and/or heteronuclear decoupling can be achieved in the detected dimension of a 2D multiple-bond correlation map, obtaining simplified cross-peaks without their characteristic fine J multiplet structure. The experimental result is a resolution improvement while the highest sensitivity is also achieved. Specifically, it is shown that the (1)H-homodecoupled band-selective (HOBS) HSQMBC experiment represents a new way to measure heteronuclear coupling constants from the simplified in-phase doublets generated along the detected dimension.

Keywords: Band-selective HSQMBC; Homonuclear decoupling; Proton–carbon coupling constants; Pure-shift; Resolution enhancement; Sensitivity improvement.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Products / chemistry*
Cyclosporine / chemistry
Magnetic Resonance Spectroscopy / methods*
Reproducibility of Results

Substances

Biological Products
Cyclosporine