Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine in DNA by selective chemical labeling

Jianlin Hu; Yuqi Chen; Xiaowei Xu; Fan Wu; Xiwen Xing; Zhenghao Xu; Jiahui Xu; Xiaocheng Weng; Xiang Zhou

doi:10.1016/j.bmcl.2013.11.017

Discrimination between 5-hydroxymethylcytosine and 5-methylcytosine in DNA by selective chemical labeling

Bioorg Med Chem Lett. 2014 Jan 1;24(1):294-7. doi: 10.1016/j.bmcl.2013.11.017. Epub 2013 Nov 18.

Authors

Jianlin Hu¹, Yuqi Chen¹, Xiaowei Xu¹, Fan Wu¹, Xiwen Xing¹, Zhenghao Xu¹, Jiahui Xu¹, Xiaocheng Weng¹, Xiang Zhou²

Affiliations

¹ College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
² College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, PR China. Electronic address: xzhou@whu.edu.cn.

PMID: 24295785
DOI: 10.1016/j.bmcl.2013.11.017

Abstract

Detection of DNA damage has been greatly improved following the development of equipment and techniques, however, discrimination between 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) is still a thorny problem. In the present study, an approach to oxidize and selective label (Ox-Labeling) 5-hmC in native DNA has been reported, which conveniently distinguishes 5-hmC from 5-mC using simple and effective processes.

Keywords: Chemoselectivity; DNA damage; Fluorescent dyes; Nucleosides; Oligodeoxyribonucleotides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5-Methylcytosine / chemistry*
Crystallography, X-Ray
Cytosine / analogs & derivatives*
Cytosine / chemistry
DNA / chemistry*
Models, Molecular
Molecular Structure

Substances

5-hydroxymethylcytosine
5-Methylcytosine
Cytosine
DNA