A facile, sensitive and selective fluorescent probe for heparin based on aggregation-induced emission

Talanta. 2014 Jan:118:348-52. doi: 10.1016/j.talanta.2013.09.055. Epub 2013 Oct 8.

Abstract

A facile, rapidly responsive fluorescence turn-on probe for heparin with high selectivity and sensitivity was reported in this paper. The probe could aggregate on the negatively charged heparin template through electrostatic interactions and then display intense fluorescence due to its aggregation-induced emission (AIE) characteristics. Under optimal condition, the probe showed high selectivity to heparin over chondroitin sulfate(ChS), hyaluronic acid (HA), dextran (DeX) and other substances, with a linear range of 0.2-14 μg/mL, and a detection limit of 57.6 ng/mL. In diluted serum, it also showed good performance.

Keywords: Aggregation-induced emission; Electrostatic interaction; Heparin; Positively charged salicylaldehyde azine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chondroitin Sulfates / analysis
  • Chondroitin Sulfates / chemistry
  • Dextrans / analysis
  • Dextrans / chemistry
  • Fluorescent Dyes / chemistry*
  • Heparin / analysis*
  • Heparin / chemistry*
  • Hyaluronic Acid / analysis
  • Hyaluronic Acid / chemistry
  • Limit of Detection
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence

Substances

  • Dextrans
  • Fluorescent Dyes
  • Hyaluronic Acid
  • Heparin
  • Chondroitin Sulfates