Colorimetric detection of hepatitis E virus based on reverse transcription loop mediated isothermal amplification (RT-LAMP) assay

J Virol Methods. 2014 Mar:197:29-33. doi: 10.1016/j.jviromet.2013.11.006. Epub 2013 Nov 21.

Abstract

Hepatitis E virus (HEV) infection is a common communicable disease in the third world countries. The early diagnosis of HEV is important for the prognosis and prevention of the spread of the disease. Conventional methods for HEV detection need expensive equipment and cannot meet the needs of point of care (POC) use. In the present study, a gold nanoparticle (AuNP) based RT-LAMP assay was developed for colorimetric detection of HEV. As low as 10(1) copies of HEV RNA were detected visually. With reduced handling and less equipment requirement, this assay provided a simple and rapid alternative to other available methods.

Keywords: Colorimetric detection; Hepatitis E virus; Loop mediated isothermal amplification.

Publication types

  • Evaluation Study

MeSH terms

  • Colorimetry / methods*
  • Gold
  • Hepatitis E / diagnosis*
  • Hepatitis E / virology
  • Hepatitis E virus / isolation & purification*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Nanoparticles
  • Nucleic Acid Amplification Techniques / methods*
  • Reverse Transcription

Substances

  • Gold