Minor FSH surge, minor follicular wave, and resurgence of preovulatory follicle several days before ovulation in heifers

Abstract

Blood samples were collected and follicle diameters were determined daily beginning on Day 12 (Day 0 = ovulation) in 35 interovulatory intervals (IOIs) in heifers. A minor follicular wave with maximal diameter (6.0 ± 0.3 mm) on Day -4 was detected in six of seven IOIs that were scanned for follicles 4 mm or greater. The number of IOIs with a CV-identified minor FSH surge toward the end of the IOI was greater (P < 0.03) in two-wave IOIs (10/17) than in three-wave IOIs (4/18). The 17 two-wave IOIs were used for study of the temporal relationships among preovulatory follicle, FSH, LH, and estradiol. Daily growth rate of the preovulatory follicle was maximum on Days -11 to -7, minimum (P < 0.05) on Days -7 to -4, and increased (resurged, P < 0.05) on Days -4 to -3. A transient increase in FSH was maximum on mean Day -4, and the peak of a minor FSH surge occurred on Day -4.5 ± 0.2. Concentration of LH and estradiol increased between Days -5 and -4. Results demonstrated resurgence of the preovulatory follicle apparently for the first time in any species. Resurgence seemed more related temporally to the minor FSH surge than to the LH increase, but further study is needed. Results supported the novel hypotheses that a minor FSH surge near the end of the IOI is temporally associated with (1) the emergence of a minor follicular wave and (2) the resurgence in growth rate of the preovulatory follicle.

Keywords: Estradiol; Luteinizing hormone; Minor FSH surge; Minor follicular wave; Resurgence of preovulatory follicle.