A modified technique for efficient radiolabeling of 68Ga-citrate from a SnO2-based 68Ge/68Ga generator for better infection imaging

Hell J Nucl Med. 2013 Sep-Dec;16(3):193-8.

Abstract

Our aim was to develop a practical method to prepare 68Ga-citrate using a SnO2-based 68Ge/68Ga generator and evaluate its use in infection imaging. 68Ga-citrate synthesis was performed in a straight forward, quantitative, one-step-aseptic procedure; an amended labeling method was applied using ACD-A buffered citrate as a precursor. Study participants were imaged on a Siemens Biograph 40 PET/CT scanner 60 min post intravenous injection. Our results showed: 90%-95% 68Ga-yield was obtained and subsequently used at 324-527 MBq to perform three to four parallel 68Ga-citrate syntheses. 68Ga-citrate of 96%-99% was yielded after 10 min incubation. The radiochemical purity was >99% with a pH value of 4.0-4.5. All other quality control requirements were met. The 68Ga-citrate stability was >96%. The final product was sterile, pyrogen and solvent-free, with very low 68Ge-levels, with 191±33 MBq in 6.6±2.8 mL. High quality images were obtained at 60 min post injection of 185 MBq of 68Ga-citrate. In conclusion, a fast, direct and cheap method with a quantitative preparation of 68Ga-citrate was described. We reported on the adaptations needed when using a SnO2-based 68Ge/68Ga generator and ACD-A buffered citrate as a precursor. This method allowed for multiple patient productions from one generator elution, with 300 MBq/patient of 68Ga-citrate produced in less than 30 min and excellent labeling reproducibility for routine infection imaging.

MeSH terms

  • Citrates / chemical synthesis*
  • Gallium*
  • Germanium / chemistry*
  • Humans
  • Infections / diagnostic imaging*
  • Inflammation / diagnostic imaging*
  • Isotope Labeling / methods
  • Neoplasms / diagnostic imaging*
  • Radioisotopes / chemistry
  • Radionuclide Imaging
  • Radiopharmaceuticals / chemical synthesis
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tin Compounds / chemistry*

Substances

  • Citrates
  • Radioisotopes
  • Radiopharmaceuticals
  • Tin Compounds
  • Germanium
  • Gallium
  • gallium citrate
  • stannic oxide