A special shear thinning phenomenon followed by static self-thickening in chitosan-graft-polyacrylamide (GPAM) aqueous solutions was investigated. This multiregion shear thinning can be defined as the first stage of the recently reported shear induced self-thickening (SIT) in our previous work. The three thinning regions (labeled as N1, N2, and N3) are considered very important, and they can reflex the complex variations of intermolecular interactions among and inside the aggregates in solution with increasing shear rate. To verify this multiregion shear thinning, a critical concentration of GPAM for this three-region shear thinning was first investigated. Shear recovery tests with the maximal shear rates located in the N1-N3 were carried out to ascertain the crucial role of shear thinning in SIT. The mechanisms of these three shear thinning regions were proposed based on the dependence of shear rheological behavior on various conditions in each region, including GPAM concentration, grafting ratio, temperature, added hydrogen bonding breaker, and salt. The above results confirm that N1 is due to the breakage of the interactions among hydrogen bonding aggregates, while N2 and N3 are attributed to the progressive destruction of the aggregates. As the first stage of SIT, shear thinning can markedly break the original aggregate and expose additional hydrogen bonding stickers to reform more aggregates with bigger size, resulting in the final higher viscosity.