A blueprint for cost-efficient localization microscopy

Chemphyschem. 2014 Mar 17;15(4):651-4. doi: 10.1002/cphc.201300739. Epub 2013 Nov 13.

Abstract

Crystal clear: The authors introduce a miniaturized localization microscopy setup based on cost-effective components. They demonstrate its feasibility for subdiffraction resolution fluorescence imaging in resolving different cellular nanostructures. The setup can be used advantageously in practical courses for training students in super-resolution fluorescence microscopy.

Keywords: cost-efficiency; fluorescence; laser spectroscopy; localization microscopy; super-resolution imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Humans
  • Image Processing, Computer-Assisted / economics*
  • Image Processing, Computer-Assisted / instrumentation
  • Microscopy, Fluorescence / economics*
  • Microscopy, Fluorescence / instrumentation
  • Software