One cultivar ('GR876') and two advanced Ohio soft red winter wheat lines ('OH413' and 'OH414'), with 'Kavkaz' in their pedigrees, were examined for the presence of the 'Kavkaz,' 1RS/1BL rye/wheat chromosome translocation. Another advanced line ('OH416'), with 'Amigo' in its pedigree, was examined for the presence of the 'Amigo,' 1RS/1AL translocation. Only two satellited chromosomes were observed in most mitotic root-tip cells from 'GR876,' 'OH413,' and 'OH414,' compared to four in most cells from 'OH416.' Heteromorphic bivalents were observed in most PMCs from hybrids produced by crossing 'GR876,' 'OH413,' and 'OH414' as females to 'Chinese Spring.' No heteromorphic bivalents were observed in PMCs from 'OH416' x 'Chinese Spring' hybrids. When 'GR876' and the Ohio lines were hybridized with 'Chinese Spring' dimonotelosomic-1B, telosomic trivalents, consisting of the short- and longarm telosomes paired with chromosome 1B, were only observed in PMCs from 43-chromosome hybrids involving 'OH416.' The long-arm telosome paired with the translocation chromosome, while the short-arm telosome remained unpaired in all other 43-chromosome hybrids. Separation of gliadin proteins from 'GR876' and the Ohio lines by PAGE revealed that secalin bands for 'GR876,' 'OH413,' and 'OH414,' migrated similarly to the secalins for 'Kavkaz.' Bands for 'OH416,' identified as possible secalins, migrated similarly to those for 'Amigo.' Cultivar 'GR876' and advanced Ohio soft red winter wheat lines 'OH413' and 'OH414' carry the 'Kavkaz' translocation, while 'OH416' carries the 'Amigo' translocation.