Abstract
Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, we examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1 we observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that we identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's function in Snc1 recycling. Our finding of genetic interactions of VPS1 with genes involved in early endosome-to-Golgi traffic further suggests Vps1 functions as a recycling factor in the membrane traffic. Finally, we provide evidence that the severe accumulation of GFP-Snc1 cytoplasmic puncta in vps1Δ cells is attributed to a mild defect in the retention of the GARP component Vps51 at the late Golgi, as well as a severe disruption of actin cables.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Actins / genetics
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Actins / metabolism
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Actins / ultrastructure
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Biological Transport
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Cell Membrane / metabolism
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Endocytosis
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Endosomes / metabolism*
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GTP-Binding Proteins / deficiency
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GTP-Binding Proteins / genetics*
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Gene Expression Regulation, Fungal*
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Genes, Reporter
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Golgi Apparatus / metabolism*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Protein Structure, Tertiary
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R-SNARE Proteins / genetics
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R-SNARE Proteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Signal Transduction
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Vesicular Transport Proteins / deficiency
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Vesicular Transport Proteins / genetics*
Substances
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Actins
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R-SNARE Proteins
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SNC1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Vesicular Transport Proteins
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Green Fluorescent Proteins
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GTP-Binding Proteins
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VPS1 protein, S cerevisiae