[Sequencing and analysis of the complete mitochondrial DNA of Chinese moccasin for medicinal use]

Nian Chen; Shen-Gan Gao; Xiao-Ping Lai; Peng Liu; Xin Wang; Ling-Li Wang

[Sequencing and analysis of the complete mitochondrial DNA of Chinese moccasin for medicinal use]

Zhong Yao Cai. 2013 May;36(5):711-5.

[Article in Chinese]

Authors

Nian Chen¹, Shen-Gan Gao, Xiao-Ping Lai, Peng Liu, Xin Wang, Ling-Li Wang

Affiliation

¹ Zhongshan Torch Polytechnic, Zhongshan 528436, China.

PMID: 24218958

Abstract

Objective: To sequence and analyze the complete mitochondrial DNA of Chinese moccasin.

Methods: The circular 17 541 bp sequence of mitochondrial DNA (mtDNA) of Chinese moccasin was determined by Ex-Taq PCR, TA-cloning and primer-walking methods.

Results: This mitogenome contained 37 coding genes (including 22 tRNA, 2 rRNA and 13 protein-coding genes) and two control regions (CR and psiCR). The gene content and arrangement of Chinese moccasin mtDNA was similar to those of other vipers reported so far.

Conclusion: The characteristics of the replication origin and the tRNA arrangement of snake mitochondrial genomes can be used for identification of medicinal snakes.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
DNA, Mitochondrial / genetics
Genome, Mitochondrial / genetics*
Molecular Sequence Data
Phylogeny*
Polymerase Chain Reaction
RNA, Ribosomal / genetics
RNA, Transfer / genetics
Sequence Analysis, DNA
Snakes / genetics*

Substances

DNA, Mitochondrial
RNA, Ribosomal
RNA, Transfer