Crambescidin-816 acts as a fungicidal with more potency than crambescidin-800 and -830, inducing cell cycle arrest, increased cell size and apoptosis in Saccharomyces cerevisiae

Mar Drugs. 2013 Nov 8;11(11):4419-34. doi: 10.3390/md11114419.

Abstract

In this paper, we show the effect of crambescidin-816, -800, and -830 on Saccharomyces cerevisiae viability. We determined that, of the three molecules tested, crambescidin-816 was the most potent. Based on this result, we continued by determining the effect of crambescidin-816 on the cell cycle of this yeast. The compound induced cell cycle arrest in G2/M followed by an increase in cell DNA content and size. When the type of cell death was analyzed, we observed that crambescidin-816 induced apoptosis. The antifungal effect indicates that crambescidins, and mostly crambescidin-816, could serve as a lead compound to fight fungal infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / pharmacology*
  • Apoptosis / drug effects
  • Cell Cycle Checkpoints / drug effects*
  • Cell Death / drug effects
  • Cell Division / drug effects
  • Cell Size / drug effects
  • Fungicides, Industrial / pharmacology*
  • G2 Phase / drug effects
  • Guanidine / analogs & derivatives
  • Guanidine / pharmacology
  • Membrane Potential, Mitochondrial / drug effects
  • Saccharomyces cerevisiae / drug effects*
  • Spiro Compounds / pharmacology*

Substances

  • Alkaloids
  • Fungicides, Industrial
  • Spiro Compounds
  • crambescidin 800
  • crambescidin 816
  • Guanidine