Abstract
We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / analysis*
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Adenosine Triphosphate / chemistry
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Animals
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Aptamers, Nucleotide / chemistry
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Cell Line, Tumor
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Coordination Complexes / chemistry*
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G-Quadruplexes*
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Iridium / chemistry*
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Luminescence
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Luminescent Measurements / methods
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Mice
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Molecular Structure
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Reproducibility of Results
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Spectrometry, Fluorescence
Substances
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Aptamers, Nucleotide
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Coordination Complexes
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Iridium
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Adenosine Triphosphate
Grants and funding
This work is supported by Hong Kong Baptist University (FRG2/11-12/009, FRG2/12-13/021), Centre for Cancer and Inflammation Research, School of Chinese Medicine (CCIR-SCM, HKBU), the Health and Medical Research Fund (HMRF/11101212), the Research Grants Council (HKBU/201811 and HKBU/204612), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), the Science and Technology Development Fund, Macao SAR (001/2012/A) and the University of Macau (MYRG091(Y2-L2)-ICMS12-LCH and MYRG121(Y2-L2)-ICMS12-LCH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.