Plant regeneration from protoplast cultures of Passiflora edulis var. flavicarpa Deg., P. amethystina Mikan. and P. cincinnata Mast

M C Dornelas; M L Vieira

doi:10.1007/BF00235300

Plant regeneration from protoplast cultures of Passiflora edulis var. flavicarpa Deg., P. amethystina Mikan. and P. cincinnata Mast

Plant Cell Rep. 1993 Dec;13(2):103-6. doi: 10.1007/BF00235300.

Authors

M C Dornelas¹, M L Vieira

Affiliation

¹ Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Caixa Postal 83, 13418-900, Piracicaba, SP, Brasil.

PMID: 24196297
DOI: 10.1007/BF00235300

Abstract

Protoplasts isolated from seedling cotyledons of yellow passionfruit (Passiflora edulis var. flavicarpa Deg.) and two related wild species, P. amethystina Mikan. and P. cincinnata Mast., divided in culture and produced calli. Shoot regeneration was obtained in MS medium (Murashige and Skoog 1962) containing 2.0 mg/l 6-benzylaminopurine (BAP). Regenerated plants produced roots in half-strength hormone-free MS medium and could be transferred to soil after being acclimatized.