A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leaves and flowers after another 4 to 8 weeks in culture. Administration of compressed air at a 300 ml/min flow rate for 30 min 10 or 15 times a day induced the development of parthenocarpic fruits from flowers. Fruits, up to 170 mm long by 35 mm diameter, were obtained within 30 to 45 d after flower opening.