Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry

Abstract

The purpose of this protocol is to take any biopsy or whole organ tissue from animals or human, formalin-fix the specimen to preserve the current state of the tissue, and embed it into a paraffin block and for future immunohistochemistry experiments (If you intend to fix cells, check the alternative protocols: Preparation of Cells for Microscopy using Cytospin, Preparation of Cells for Microscopy using Chamber Slides and Coverslips, or Preparation of Cells for Microscopy using 'Cell Blocks').

Keywords: Fixation of tissue; Formalin-fixed paraffin-embedded tissue (FFPET); Immunohistochemistry; Tissue process and embedded; Tissue section.