Inheritance of restriction fragment length polymorphisms and random amplified polymorphic DNAs in coastal Douglas-fir

K D Jermstad; A M Reem; J R Henifin; N C Wheeler; D B Neale

doi:10.1007/BF00223716

Inheritance of restriction fragment length polymorphisms and random amplified polymorphic DNAs in coastal Douglas-fir

Theor Appl Genet. 1994 Nov;89(6):758-66. doi: 10.1007/BF00223716.

Authors

K D Jermstad¹, A M Reem, J R Henifin, N C Wheeler, D B Neale

Affiliation

¹ Pacific Southwest Research Station, USDA Forest Service, Institute of Forest Genetics, 2480 Carson Road, 95667, Placerville, CA, USA.

PMID: 24178022
DOI: 10.1007/BF00223716

Abstract

A total of 225 new genetic loci [151 restriction fragment length polymorphisms (RFLP) and 74 random amplified polymorphic DNAs (RAPD)] in coastal Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco var. menziesii] have been identified using a three-generation outbred pedigree. The Mendelian inheritance of 16 RFLP loci and 29 RAPD loci was demonstrated based on single-locus segregation in a sample of F2 progeny. One RFLP locus, PtIFG2025, showed segregation distortion. Probe pPtIFG2025 is a loblolly pine cDNA probe encoding for rbcS. The 16 RFLP loci and 23 allozyme loci were also assayed in a sample of 16 Douglas-fir seed-orchard clones. Allelism was determined at 11 of the 16 RFLP loci. RFLPs were able to detect slightly more variation (4.0 alleles per locus) than allozymes (3.1 alleles per locus). The inheritance of an additional 80 RAPD loci was determined based on haploid segregation analysis of megagametophytes from parent tree 013-1. Once 200-300 markers are identified and placed on a genetic map, quantitative trait loci affecting bud phenology will be mapped.