Abstract
Illumina microarray was used to identify differentially expressed genes in three epithelial ovarian cancer (EOC) cells. To validate the microarray data, mRNA and protein level of glucose transporter-1 (GLUT-1) was examined. GLUT-1 had an EOC/normal cells ratio of 5.51 based on microarray. Real-time PCR and immunohistochemistry demonstrated that GLUT-1 expression was significantly increased in EOC (p = .029 and p < .001, respectively). On survival analysis, GLUT-1 overexpression (HR = 4.80, p = .027) and lymph node metastases (HR = 8.35, p = .016) conferred a significantly worse overall survival. In conclusion, GLUT-1 expression is remarkably upregulated in EOC and predicts a poor overall survival.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism*
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Carcinoma, Ovarian Epithelial
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Cell Line, Tumor
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Female
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Gene Expression Profiling / methods
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Glucose Transporter Type 1 / genetics
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Glucose Transporter Type 1 / metabolism*
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Humans
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Immunohistochemistry
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Kaplan-Meier Estimate
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Lymphatic Metastasis
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Neoplasms, Glandular and Epithelial / genetics
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Neoplasms, Glandular and Epithelial / metabolism*
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Neoplasms, Glandular and Epithelial / mortality
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Neoplasms, Glandular and Epithelial / secondary
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Oligonucleotide Array Sequence Analysis
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Ovarian Neoplasms / genetics
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Ovarian Neoplasms / metabolism*
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Ovarian Neoplasms / mortality
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Ovarian Neoplasms / pathology
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Ovarian Neoplasms / secondary
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Predictive Value of Tests
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Prognosis
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RNA, Messenger / metabolism
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Real-Time Polymerase Chain Reaction
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Reproducibility of Results
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Time Factors
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Up-Regulation
Substances
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Biomarkers, Tumor
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Glucose Transporter Type 1
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RNA, Messenger
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SLC2A1 protein, human