Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana, into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation

Biotechnol Lett. 2014 Mar;36(3):575-9. doi: 10.1007/s10529-013-1386-0. Epub 2013 Oct 27.

Abstract

Aureochrome-1 (AUREO1) is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida. We have cloned the gene, NgAUREO1, coding for AUREO1 from Nannochloropsis gaditana, and report that the lipid content in recombinant Saccharomyces cerevisiae was 1.6-fold more than in wild-type S. cerevisiae (6.3 % lipid increased to 10 %). Over-expression of AUREO1 in S. cerevisiae up-regulated the expression of acetyl-CoA carboxylase and acyl-CoA:diacylglycerol acyl-transferase but down-regulated the expression of long-chain-acyl CoA synthetase. This enhanced the accumulation of lipid. This study highlights a novel function of AUREO1 and allows a better understanding of the regulation mechanism of fatty acid metabolism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA Carboxylase / biosynthesis
  • Coenzyme A Ligases / biosynthesis
  • Diacylglycerol O-Acyltransferase / biosynthesis
  • Gene Expression
  • Gene Expression Profiling
  • Lipid Metabolism*
  • Lipids / analysis*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Stramenopiles / genetics*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Lipids
  • Recombinant Proteins
  • Transcription Factors
  • Diacylglycerol O-Acyltransferase
  • Coenzyme A Ligases
  • Acetyl-CoA Carboxylase