Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification

Kevin W Soli; Monalisa Kas; Tobias Maure; Masahiro Umezaki; Ayako Morita; Peter M Siba; Andrew R Greenhill; Paul F Horwood

doi:10.1016/j.diagmicrobio.2013.09.009

Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification

Diagn Microbiol Infect Dis. 2013 Dec;77(4):321-3. doi: 10.1016/j.diagmicrobio.2013.09.009. Epub 2013 Sep 28.

Authors

Kevin W Soli¹, Monalisa Kas, Tobias Maure, Masahiro Umezaki, Ayako Morita, Peter M Siba, Andrew R Greenhill, Paul F Horwood

Affiliation

¹ Papua New Guinea Institute of Medical Research, Goroka, Papua New Guinea.

PMID: 24157057
DOI: 10.1016/j.diagmicrobio.2013.09.009

Abstract

We evaluated loop-mediated isothermal amplification end-point detection methods for Salmonella, Shigella, and Vibrio cholerae. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.

Keywords: Colorimetric detection; Hydroxynaphthol blue; LAMP; Loop-mediated isothermal amplification; SYBR Green; Salmonella; Shigella; Vibrio cholerae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry / methods*
Humans
Molecular Typing
Nucleic Acid Amplification Techniques* / methods
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Salmonella / classification
Salmonella / genetics*
Sensitivity and Specificity
Shigella / classification
Shigella / genetics*
Vibrio cholerae / classification
Vibrio cholerae / genetics*