3D static elastography at the micrometer scale using Full Field OCT

Amir Nahas; Morgane Bauer; Stéphane Roux; A Claude Boccara

doi:10.1364/BOE.4.002138

3D static elastography at the micrometer scale using Full Field OCT

Biomed Opt Express. 2013 Sep 12;4(10):2138-49. doi: 10.1364/BOE.4.002138. eCollection 2013.

Authors

Amir Nahas¹, Morgane Bauer, Stéphane Roux, A Claude Boccara

Affiliation

¹ Institut Langevin, ESPCI, 1 rue Jussieu, 75005 Paris, France ; LLTech, Pépinière Paris Sant Cochin 29 rue du Faubourg Saint Jacques 75014 Paris, France.

Abstract

Full-Field OCT (FF-OCT) is able to image biological tissues in 3D with micrometer resolution. In this study we add elastographic contrast to the FF-OCT modality. By combining FF-OCT with elastography, we create a virtual palpation map at the micrometer scale. We present here a proof of concept on multi-layer phantoms and preliminary results on ex vivo biological samples such as porcine cornea, human breast tissues and rat heart. The 3D digital volume correlation that is used in connection with the 3D stack of images allows to access to the full 3D strain tensor and to reveal stiffness anisotropy.

Keywords: (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography; (180.0180) Microscopy; (180.1655) Coherence tomography; (180.3170) Interference microscopy; (180.6900) Three-dimensional microscopy.