Minimum set of cytochromes P450 for reconstituting the biosynthesis of camalexin, a major Arabidopsis antibiotic

Angew Chem Int Ed Engl. 2013 Dec 16;52(51):13625-8. doi: 10.1002/anie.201307454. Epub 2013 Oct 21.

Abstract

Bringing it all together: The missing key step in the biosynthesis of camalexin was uncovered by in vitro biochemical characterization. The coupling of Trp- and Cys-derived fragments through CS bond formation is promoted by an unusual cytochrome P450 CYP71A13. The in vitro reconstitution of the camalexin biosynthesis (left) from Trp and Cys was achieved using just three cytochromes P450. IAN=indole-3-acetonitrile.

Keywords: CS bond formation; biosynthesis; camalexin; cytochromes; natural products.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Arabidopsis
  • Arabidopsis Proteins / metabolism*
  • Biological Products
  • Cytochrome P-450 Enzyme System / metabolism*
  • Indoles / chemical synthesis*
  • Indoles / chemistry
  • Thiazoles / chemical synthesis*
  • Thiazoles / chemistry

Substances

  • Arabidopsis Proteins
  • Biological Products
  • Indoles
  • Thiazoles
  • camalexin
  • Cytochrome P-450 Enzyme System