Anti-listeria activity of poly(lactic acid)/sawdust particle biocomposite film impregnated with pediocin PA-1/AcH and its use in raw sliced pork

Int J Food Microbiol. 2013 Oct 15;167(2):229-35. doi: 10.1016/j.ijfoodmicro.2013.09.009. Epub 2013 Sep 21.

Abstract

A novel poly(lactic acid) (PLA)/sawdust particle (SP) biocomposite film with anti-listeria activity was developed by incorporation of pediocin PA-1/AcH (Ped) using diffusion coating method. Sawdust particle played an important role in embedding pediocin into the hydrophobic PLA film. The anti-listeria activity of the PLA/SP biocomposite film incorporated with Ped (PLA/SP+Ped) was detected, while no activity against the tested pathogen was observed for the control PLA films (without SP and/or Ped). Dry-heat treatment of film before coating with Ped resulted in the highest Ped adsorption (11.63 ± 3.07 μg protein/cm(2)) and the highest anti-listeria activity. A model study of PLA/SP+Ped as a food-contact antimicrobial packaging on raw sliced pork suggests a potential inhibition of Listeria monocytogenes (99% of total listerial population) on raw sliced pork during the chilled storage. This study supports the feasibility of using PLA/SP+Ped film to reduce the initial load of L. monocytogenes on the surface of raw pork.

Keywords: Anti-listeria; Biocomposite film; Packaging; Pediocin; Poly(lactic acid); Sawdust particle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Infective Agents / pharmacology
  • Bacteriocins / pharmacology*
  • Food Contamination / prevention & control
  • Food Packaging*
  • Lactic Acid / pharmacology*
  • Listeria monocytogenes / drug effects*
  • Meat / microbiology*
  • Microbial Sensitivity Tests
  • Pediocins
  • Polyesters
  • Polymers / pharmacology*
  • Solubility
  • Swine
  • Tensile Strength

Substances

  • Anti-Infective Agents
  • Bacteriocins
  • Pediocins
  • Polyesters
  • Polymers
  • pediocin PA-1
  • Lactic Acid
  • poly(lactide)