Abstract
Cervical cancer lacks reliable prognostic factors for both progression and chemotherapeutic responsiveness. The expression of the LDOC1 tumor suppressor candidate was therefore investigated. In four of six cervical cancer cell lines tested, expression of LDOC1 was silenced. Downregulation of LDOC1 could also be shown in biopsies of cervical cancer specimens. PCR-based promoter methylation analysis revealed a significant association between promoter methylation and the loss of LDOC1 expression, which could be reverted by DNA methyltransferase inhibitors. This indicates that silencing of LDOC1 is a frequent event in cervical cancer and may be of interest as a molecular marker in cervical cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biopsy
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Cell Death
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DNA Methylation*
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DNA Modification Methylases / antagonists & inhibitors
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DNA Modification Methylases / metabolism
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Down-Regulation
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Enzyme Inhibitors / pharmacology
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Female
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Gene Expression Regulation, Neoplastic
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Gene Silencing
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HeLa Cells
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Humans
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Promoter Regions, Genetic*
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Transfection
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Tumor Suppressor Proteins / genetics*
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Tumor Suppressor Proteins / metabolism
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Uterine Cervical Neoplasms / genetics*
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Uterine Cervical Neoplasms / metabolism
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Uterine Cervical Neoplasms / pathology
Substances
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Enzyme Inhibitors
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LDOC1 protein, human
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Nuclear Proteins
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Tumor Suppressor Proteins
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DNA Modification Methylases