Photoactivation of a psoralen-blocked luciferase gene by blue light

Angew Chem Int Ed Engl. 2013 Nov 18;52(47):12448-51. doi: 10.1002/anie.201306150. Epub 2013 Oct 2.

Abstract

A single psoralen cross-link completely blocks expression of a gene. An aminopyrene derivative has been found that allows the efficient light-triggered activation of cross-linked genes by UV or blue light. This paves the way to apply such psoralen cross-links for the photocleavable protection of nucleic acids.

Keywords: electron transfer; nucleic acids; photoactivation; photochemistry; pyrene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / metabolism
  • DNA Adducts / chemistry
  • Electron Transport
  • Ficusin / chemistry*
  • Light
  • Luciferases / chemistry
  • Luciferases / genetics*
  • Luciferases / metabolism
  • Protective Agents / chemistry
  • Protective Agents / metabolism
  • Ultraviolet Rays

Substances

  • DNA Adducts
  • Protective Agents
  • DNA
  • Luciferases
  • Ficusin