In this work, a novel approach to 3-dimensional (3D) electrode fabrication, based on electrodeposited polypyrrole (PPy), for dielectrophoresis (DEP) is described. 3D PPy electrodes with post and cage geometries were grown over planar interdigitated electrodes. Computational modelling and experimental work were carried out to assess the performance of the proposed electrode geometries. It was found that these new electrode geometries enhanced the dielectrophoretic trapping efficiency for polystyrene beads by exhibiting larger variations of the electric field and by affecting a larger volume of the fluid sample than planar electrodes. Applications of this work include, but are not limited to, environmental monitoring, food safety control, clinical analysis, and clean energy production.