Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

Giin-Yu Amy Tan; Chia-Lung Chen; Liya Ge; Ling Li; Lin Wang; Lei Zhao; Yu Mo; Swee Ngin Tan; Jing-Yuan Wang

doi:10.1016/j.jbiosc.2013.08.020

Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis

J Biosci Bioeng. 2014 Mar;117(3):379-82. doi: 10.1016/j.jbiosc.2013.08.020. Epub 2013 Oct 10.

Authors

Giin-Yu Amy Tan¹, Chia-Lung Chen², Liya Ge², Ling Li², Lin Wang³, Lei Zhao³, Yu Mo³, Swee Ngin Tan⁴, Jing-Yuan Wang⁵

Affiliations

¹ Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: amytangy@ntu.edu.sg.
² Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
³ Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
⁴ Natural Sciences and Science Education Academic Group, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore.
⁵ Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address: jywang@ntu.edu.sg.

PMID: 24119486
DOI: 10.1016/j.jbiosc.2013.08.020

Abstract

A gas chromatography-mass spectrometry method for quantification of polyhydroxyalkanoates (PHAs), containing 4-carbon to 16-carbon monomers, even in the absence of standards, was developed. Strong linear correlations existed between PHA carbon number and retention time/response factor (R(2) ≥ 0.987). Based on the correlations, high recovery values, between 100.5% and 114.3%, were obtained for PHA polymers.

Keywords: 3-Hydroxyalkanoic acid; Gas chromatography; Homologous; Mass spectrometry; Polyhydroxyalkanoate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / chemistry
Carbon / chemistry*
Gas Chromatography-Mass Spectrometry / methods*
Polyhydroxyalkanoates / analysis*

Substances

Polyhydroxyalkanoates
Carbon