Identification of mureidomycin analogues and functional analysis of an N-acetyltransferase in napsamycin biosynthesis

Chembiochem. 2013 Nov 25;14(17):2248-55. doi: 10.1002/cbic.201300287. Epub 2013 Oct 2.

Abstract

Antibiotic abundance: Several new uridyl peptide antibiotics were identified from a heterologous producer strain containing the mureidomycin/napsamycin biosynthetic gene cluster by using HRMS and LC-ESI-MS/MS. Analysis of the new compounds and the corresponding gene cluster revealed NpsB, an N-acetyltransferase, to be responsible for acetylation of the uridyl peptide antibiotic.

Keywords: N-acetylation; biosynthesis; heterologous expression; uridyl peptide antibiotics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Acetyltransferases / metabolism*
  • Molecular Conformation
  • Multigene Family
  • Nucleosides / biosynthesis
  • Nucleosides / chemistry
  • Nucleosides / metabolism
  • Streptomyces / enzymology
  • Streptomyces / genetics
  • Streptomyces / metabolism

Substances

  • Nucleosides
  • mureidomycin F
  • Acetyltransferases