Noninvasive cellular quantity measurement in Bone Marrow Stromal Cells/ Beta-tricalcium phosphate

Annu Int Conf IEEE Eng Med Biol Soc. 2013:2013:2048-51. doi: 10.1109/EMBC.2013.6609934.

Abstract

This paper describes noninvasive cellular quantity measurement in Bone Marrow Stromal Cells/ β-tricalcium phosphate. We attempt to identify cellular quantity with an ultrasonic system. The ultrasonic waves are reflected at boundaries where there is a difference in acoustic impedances of the materials on each side of the boundary. Therefore, we focus on the reflected signal. From the obtained ultrasonic data, we extract two features; amplitude and frequency. Amplitude is obtained from the raw ultrasonic wave, and frequency is calculated from frequency spectrum obtained by applying cross-spectrum method. Therefore, we suggest the superiority of frequency to analyze Bone Marrow Stromal Cells. This study shows the ability of intervention to produce the desired beneficial effect.

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Calcium Phosphates / chemistry*
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Tissue Engineering / methods
  • Ultrasonics / instrumentation
  • Ultrasonics / methods

Substances

  • Calcium Phosphates
  • beta-tricalcium phosphate