The title compound, C25H26Cl2FN3O4S, contains two bio-active moieties (thio-barbituric acid and fluoro-quinolone). In the crystal, mol-ecules are linked via C-H⋯O and C-H⋯F hydrogen bonds, forming two-dimensional slab-like networks lying parallel to the bc plane. The benzene ring substituted by F and Cl atoms and the 4-chloro-butyl group seem to be partly disordered, however attempts to model the disorder were unsuccessful.