Synthetic Fourier transform light scattering

Opt Express. 2013 Sep 23;21(19):22453-63. doi: 10.1364/OE.21.022453.

Abstract

We present synthetic Fourier transform light scattering, a method for measuring extended angle-resolved light scattering (ARLS) from individual microscopic samples. By measuring the light fields scattered from the sample plane and numerically synthesizing them in Fourier space, the angle range of the ARLS patterns is extended up to twice the numerical aperture of the imaging system with unprecedented sensitivity and precision. Extended ARLS patterns of individual microscopic polystyrene beads, healthy human red blood cells (RBCs), and Plasmodium falciparum-parasitized RBCs are presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Erythrocytes / cytology*
  • Erythrocytes / parasitology*
  • Fourier Analysis
  • Humans
  • Light
  • Nephelometry and Turbidimetry / methods*
  • Plasmodium falciparum / cytology*
  • Scattering, Radiation