Type I J-domain NbMIP1 proteins are required for both Tobacco mosaic virus infection and plant innate immunity

Yumei Du; Jinping Zhao; Tianyuan Chen; Qi Liu; Haili Zhang; Yan Wang; Yiguo Hong; Fangming Xiao; Ling Zhang; Qianhua Shen; Yule Liu

doi:10.1371/journal.ppat.1003659

Type I J-domain NbMIP1 proteins are required for both Tobacco mosaic virus infection and plant innate immunity

PLoS Pathog. 2013;9(10):e1003659. doi: 10.1371/journal.ppat.1003659. Epub 2013 Oct 3.

Authors

Yumei Du¹, Jinping Zhao, Tianyuan Chen, Qi Liu, Haili Zhang, Yan Wang, Yiguo Hong, Fangming Xiao, Ling Zhang, Qianhua Shen, Yule Liu

Affiliation

¹ MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.

Abstract

Tm-2² is a coiled coil-nucleotide binding-leucine rich repeat resistance protein that confers durable extreme resistance against Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) by recognizing the viral movement protein (MP). Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2² and SGT1. Silencing of NbMIP1s reduced TMV movement and compromised Tm-2²-mediated resistance against TMV and ToMV. Furthermore, silencing of NbMIP1s reduced the steady-state protein levels of ToMV MP and Tm-2². Moreover, NbMIP1s are required for plant resistance induced by other R genes and the nonhost pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. In addition, we found that SGT1 associates with Tm-2² and is required for Tm-2²-mediated resistance against TMV. These results suggest that NbMIP1s function as co-chaperones during virus infection and plant immunity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance / genetics
Disease Resistance / immunology*
Glucosyltransferases / genetics
Glucosyltransferases / immunology
Molecular Chaperones / genetics
Molecular Chaperones / immunology*
Nicotiana / genetics
Nicotiana / immunology*
Nicotiana / virology
Plant Diseases / genetics
Plant Diseases / immunology*
Plant Diseases / virology
Plant Proteins / genetics
Plant Proteins / immunology*
Protein Structure, Tertiary
Pseudomonas syringae
Tobacco Mosaic Virus / genetics
Tobacco Mosaic Virus / immunology*

Substances

Molecular Chaperones
Plant Proteins
Glucosyltransferases

Grants and funding

This work was supported by the National Basic Research Program of China (2014CB138400, 2011CB910100), the National Natural Science Foundation of China (30930060; 31270182; 31071169), the Specialized Research Fund for the Doctoral Program of Higher Education of China (20120002130005) and the National Transgenic Program of China (2011ZX08005-001, 2011ZX08010-002 and 2013ZX08009-003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.