Spectrophotometric detection of histidine and lysine using combined enzymatic reactions

Akimitsu Kugimiya; Emi Takamitsu

doi:10.1016/j.msec.2013.08.004

Spectrophotometric detection of histidine and lysine using combined enzymatic reactions

Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4867-70. doi: 10.1016/j.msec.2013.08.004. Epub 2013 Aug 15.

Authors

Akimitsu Kugimiya¹, Emi Takamitsu

Affiliation

¹ Center for Industry and Public Relations, Hiroshima City University, 3-8-24 Senda-machi, Naka-ku, Hiroshima 730-0052, Japan. Electronic address: kugimiya@hiroshima-cu.ac.jp.

PMID: 24094198
DOI: 10.1016/j.msec.2013.08.004

Abstract

An amino acid-sensing system with absorption spectrophotometric detection was developed. To ensure specific recognition of each amino acid, aminoacyl-tRNA synthetases were employed and the concentration of NADH produced by way of several enzymatic reactions was measured. Using this detection system, from 1.5 to 55 μM of histidine and from 15 to 95 μM of lysine could be measured selectively in HEPES-KOH buffer (pH 8.0).

Keywords: Amino acid; Aminoacyl-tRNA synthetase; Biosensing; Clinical examination; Food industry; β-Nicotinamide adenine dinucleotide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acyl-tRNA Synthetases / metabolism*
Histidine / analysis*
Histidine-tRNA Ligase / metabolism
Hydrogen-Ion Concentration
Lysine / analysis*
Lysine-tRNA Ligase / metabolism
NAD / metabolism
Spectrophotometry*

Substances

NAD
Histidine
Amino Acyl-tRNA Synthetases
Histidine-tRNA Ligase
Lysine-tRNA Ligase
Lysine