Analytical derivatization either in pre or post column modes is one of the most widely used sample pretreatment techniques coupled to liquid chromatography. In the present review article we selected to discuss the post column derivatization mode for the analysis of organic compounds. The first part of the review focuses to the instrumentation of post-column setups including not only fundamental components such as pumps and reactors but also less common parts such as static mixers and back-pressure regulators; the second part of the article discusses the most popular "chemistries" that are involved in post column applications, including reagent-less approaches and new sensing platforms such as the popular gold nanoparticles. Some representative recent applications are also presented as tables.
Keywords: 1,2-naphthoquinone-4-sulfonate; 2,2-diphenyl-1-picrylhydrazyl; 2,2′-azinobis-(3-ehtylbenzothiazoline-6-sulphonate; 2,3-naphthalenedicarboxaldehyde; 5,5′-dithiobis-(2-nitrobenzoic acid; 7-chloro-4-nitro-2,1,3-benzoxadiazole; ABTS; ADA; AuNPs; CL; DMAB; DPPH; DTNB; Determination; ECL; FEP; FRET; HPLC; KOT; LC; Liquid chromatography; NBD-Cl; NDA; NPs; NQS; OPA; OT; Organic compounds; PBPB; PCD; PEEK; PTFE; PVC; Post-column derivatization; RF-HPLC; Review; Ru(bpy)(3)(2+); SET; TNB; UV; anthracene-2,3-dicarboxaldehyde; chemiluminescence; dimethylaminobenzaldehyde; electrochemiluminescence; florinated ethylene propylene; fluorescence resonance energy transfer; gold nanoparticles; high pressure liquid chromatography; knitted open tubular; liquid chromatography; nanoparticles; o-phthalaldehyde; open tubular; polyetheretherketone; polytetrafluoroethylene (Teflon); polyvinyl chloride; post-column derivatization; pyridinium hydrobromide perbromide; reaction flow chromatography; single electron transfer; thiol-5-thio-2-nitrobenzoic acid; tris(2,2′-bipyridine)rythenium (III); ultraviolet.
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