A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania

Lucie Paloque; Nicolas Vidal; Magali Casanova; Aurélien Dumètre; Pierre Verhaeghe; Daniel Parzy; Nadine Azas

doi:10.1016/j.mimet.2013.09.006

A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania

J Microbiol Methods. 2013 Dec;95(3):320-3. doi: 10.1016/j.mimet.2013.09.006. Epub 2013 Sep 18.

Authors

Lucie Paloque¹, Nicolas Vidal, Magali Casanova, Aurélien Dumètre, Pierre Verhaeghe, Daniel Parzy, Nadine Azas

Affiliation

¹ Infections Parasitaires, Transmission, Physiopathologie et Thérapeutique, UMR MD3, Faculté de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, CS 30064, 13385 Marseille Cedex 05, France. Electronic address: lupaloque@hotmail.com.

PMID: 24055386
DOI: 10.1016/j.mimet.2013.09.006

Abstract

We validated a new method, based on luciferine/luciferase bioluminescence, for drug screening on promastigotes of different Leishmania species. Results obtained with this new, rapid, reproducible, and reliable method are in good accordance with results obtained by the conventional MTT assay. This bioluminescence assay has a lower detection limit.

Keywords: Accuracy; Assay validation; Leishmania; Linearity; Luminescence; Screening.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antiprotozoal Agents / isolation & purification*
Antiprotozoal Agents / pharmacology*
Drug Evaluation, Preclinical / methods*
Firefly Luciferin / metabolism
Leishmania / drug effects*
Luciferases, Firefly / analysis
Luminescent Measurements / methods*
Reproducibility of Results
Sensitivity and Specificity

Substances

Antiprotozoal Agents
Firefly Luciferin
Luciferases, Firefly